NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 15 |
| Title: | Knocking out MEK1 and MEK3 through RNAi: their function studied by transcript profiling |
| Date: | 2003-10-22 |
| Description: | The mitogen-activated protein kinase (MAPK) signal transduction pathway composed of three protein kinases in a cascade (MAPK - MEK - MEKK) is central to relay extracellular stimulus in eukaryotes. We decided to delineate MAPK pathways and learn about their function by altering specific MEK levels. We designed double stranded RNA-interference constructs using 3` UTRs of specific MEKs and placed it under a glucocorticoid-inducible expression system based on lac-repressor (from Ian Moore)and we have established Arabidopsis cell suspensions carrying these constructs. Here we suggest the transcriptomics analysis of two of these MEK RNAi lines: MEK1-RNAi and MEK3-RNAi. MEK1 was shown to interact with and activate the AtMPK4 MAPK. AtMPK4 insertional knockout was found to have a pleiotropic phenotype and microarray analysis suggested its involvement as a negative regulator of stress-responses. It will be interesting to compare this altered gene expression with our induced cell line where the putative upstream MEK1 is not present. We do not have information on the role of MEK3 so studying the mRNA level in MEK3 downregulated cells might provide the first clues to its function. Thus we plan to use microarray analysis with four samples MEK1-RNAi and MEK3-RNAi when their expression is induced or un-induced with glucocorticoid. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Bogre_A1-Bogre-wt-FI | 106 | whole seedlings | N933 | LB001_AG_A1-Bogre-wt-FI.CEL | |
| Treatment: The counterpart of this sample is a treatment with the pathogen elicitor Flagellin 22 (see sample A-2-Bogre-wt+Fl). The seedlings are transferred from plate to liquid medium at 2 leaves stage, one night before treatment. These are control seedlings, mock-treated by adding liquid culture medium to the seedlings. Seedlings were collected after 1 hour treatment. | |||||
| Bogre_A2-Bogre-wt+FI | 108 | whole seedlings | N933 | LB001_AG_A2-Bogre-wt+FI.CEL | |
| Treatment: The counterpart of this sample is a mock treatment with medium without elicitor(see sample A-1-Bogre-wt-Fl). The seedlings are transferred from plate to liquid medium at 2 leaves stage, one night before treatment. The seedlings are treated by adding flagellin in liquid medium to a final concentration of 1 microM. Samples are harvested after one hour treatment. | |||||
| Bogre_A3-Bogre-1-FI | 110 | col-0 | whole seedlings | LB001_AG_A3-Bogre-1-FI.CEL | |
| Treatment: The counterpart of this sample is a treatment with the pathogen elicitor flagellin 22 (see sample A-4-Bogre-1+Fl) on mutant plants knock out for the mapkk1 gene (mek1). The seedlings are transferred from plate to liquid medium at 2 leaves stage, one night before treatment. The seedlings are mock-treated by adding liquid medium without elicitor. Samples are harvested after one hour. | |||||
| Bogre_A4-Bogre-1+FI | 112 | col-0 | whole seedlings | LB001_AG_A4-Bogre-1+FI.CEL | |
| Treatment: The counterpart of this sample is a mock-treatment without elicitor (see sample A-3-Bogre-1-Fl) on mutant plants knock out for the mapkk1 gene (mek1). The seedlings are transferred from plate to liquid medium at 2 leaves stage, one night before treatment. The seedlings are treated by adding the elicitor in solution in culture medium to a final concentration of 1 microM. Samples are harvested after one hour of treatment. | |||||