NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:147
Title:Transcriptomic studies to understand the circadian clock temperature buffering mechanism
Date:2005-07-21
Description:The circadian clock temporally orchestrates many physiological and developmental processes. The accuracy with which it co-ordinates these events with the daily light/dark cycle gives an organism a competitive advantage. For a clock to remain accurate it must be buffered against unpredictable fluctuations in temperature. We have identified the gene GI as playing a role in this buffering process. To understand the underlying temperature buffering mechanism we aim to investigate, in gi and WT changes in the transcriptome.The Arabidopsis (wt (WS) and gi11 (WS)) seeds will be sterilised and then plated on MS 3% sucrose plates. The seeds will be cold treated for 2 days and then germinated and grown under 12h light 12h dark cycles at 22°C for 7 days. On the seventh day plants will be transferred to constant light at a range of temperatures (12°C, 17°C, 22°C and 27°C). RNA will be isolated over one circadian cycle on the third day of adaptation and then pooled, (prior to pooling the RNA will be analysed on a denaturing gel and using real time PCR and quantified using Quant-itä RNA assay). The pooling over one circadian cycle is essential when you are comparing WT to a mutant with an altered period. Using this approach we aim to identify temperature-regulated transcripts and transcripts that are mis-regulated in gi at specific temperatures.This transcriptomic study is funded by the BBSRC.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Gould_1-10_WT-WS-17degrees_Rep2_ATH12688whole seedling Gould_1-10_WT-WS-17degrees_Rep2_ATH1.CEL
Gould_1-11_WT-WS-22degrees_Rep2_ATH12689whole seedling Gould_1-11_WT-WS-22degrees_Rep2_ATH1.CEL
Gould_1-12_WT-WS-27degrees_Rep2_ATH12690whole seedling Gould_1-12_WT-WS-27degrees_Rep2_ATH1.CEL
Gould_1-13_WT-WS-12degrees_Rep3_ATH12691whole seedling Gould_1-13_WT-WS-12degrees_Rep3_ATH1.CEL
Gould_1-14_WT-WS-17degrees_Rep3_ATH12692whole seedling Gould_1-14_WT-WS-17degrees_Rep3_ATH1.CEL
Gould_1-15_WT-WS-22degrees_Rep3_ATH12693whole seedling Gould_1-15_WT-WS-22degrees_Rep3_ATH1.CEL
Gould_1-16_WT-WS-27degrees_Rep3_ATH12694whole seedling Gould_1-16_WT-WS-27degrees_Rep3_ATH1.CEL
Gould_1-17_GI11-MUT-12degrees_Rep2_ATH12695WSwhole seedling Gould_1-17_GI11-MUT-17degrees-Rep2_ATH1.CEL
Gould_1-18_GI11-MUT-17degrees_Rep2_ATH12696WSwhole seedling Gould_1-18_GI11-MUT-17degrees_Rep2_ATH1.CEL
Gould_1-19_GI11-MUT-22degrees_Rep2_ATH12697WSwhole seedling Gould_1-19_GI11-MUT-22degrees_Rep2_ATH1.CEL
Gould_1-1_WT-WS-12degrees_Rep1_ATH12478Cell culture Gould_1-1_WT-WS-12C_ATH1.CEL
Gould_1-20_GI11-MUT-27degrees_Rep2_ATH12698WSwhole seedling Gould_1-20_GI11-MUT-27degrees_Rep2_ATH1.CEL
Gould_1-21_GI11-MUT-12degrees_Rep3_ATH12699WSwhole seedling Gould_1-21_GI11-MUT-12degrees_Rep3_ATH1.CEL
Gould_1-22_GI11-MUT-17degrees_Rep3_ATH12700WSwhole seedling Gould_1-22_GI11-MUT-17degrees_Rep3_ATH1.CEL
Gould_1-23_GI11-MUT-22degrees_Rep3_ATH12701WSwhole seedling Gould_1-23_GI11-MUT-22degrees_Rep3_ATH1.CEL
Gould_1-24_GI11-MUT-27degrees_Rep3_ATH12702WSwhole seedling Gould_1-24_GI11-MUT-27degrees_Rep3_ATH1.CEL
Gould_1-2_WT-WS-17degrees_Rep1_ATH12479Cell culture Gould_1-2_WT-WS-17C_ATH1.CEL
Gould_1-3_WT-WS-22degrees_Rep1_ATH12480Cell culture Gould_1-3_WT-WS-22C_ATH1.CEL
Gould_1-4_WT-WS-27degrees_Rep1_ATH12481Cell culture Gould_1-4_WT-WS-27C_ATH1.CEL
Gould_1-5_WT-GI-12degrees_Rep1_ATH12482WSCell culture Gould_1-5_WT-GI-12C_ATH1.CEL
Gould_1-6_WT-GI-17degrees_Rep1_ATH12483WSCell culture Gould_1-6_WT-GI-17C_ATH1.CEL
Gould_1-7_WT-GI-22degrees_Rep1_ATH12484WSCell culture Gould_1-7_WT-GI-22C_ATH1.CEL
Gould_1-8_WT-GI-27degrees_Rep1_ATH12485WSCell culture Gould_1-8_WT-GI-27C_ATH1.CEL
Gould_1-9_WT-WS-12degrees_Rep2_ATH12687whole seedling Gould_1-9_WT-WS-12degrees_Rep2_ATH1.CEL