NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:146
Title:AtGenExpress:Stress Treatments (Heat stress)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana. The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wild Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-9111_Heatstress-Shoots-0.5h_Rep12450Shoots N1092AtGen_6-9111_Heatstress-Shoots-0.5h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9112_Heatstress-Shoots-0.5h_Rep22451Shoots N1092AtGen_6-9112_Heatstress-Shoots-0.5h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9121_Heatstress-Roots-0.5h_Rep12452Roots N1092AtGen_6-9121_Heatstressoots-0.5h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9122_Heatstress-Roots-0.5h_Rep22453Roots N1092AtGen_6-9122_Heatstressoots-0.5h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9211_Heatstress-Shoots-1.0h_Rep12454Shoots N1092AtGen_6-9211_Heatstress-Shoots-1.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9212_Heatstress-Shoots-1.0h_Rep22455Shoots N1092AtGen_6-9212_Heatstress-Shoots-1.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9221_Heatstress-Roots-1.0h_Rep12456Roots N1092AtGen_6-9221_Heatstressoots-1.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9222_Heatstress-Roots-1.0h_Rep22457Roots N1092AtGen_6-9222_Heatstressoots-1.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9311_Heatstress-Shoots-3.0h_Rep12458Shoots N1092AtGen_6-9311_Heatstress-Shoots-3.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9312_Heatstress-Shoots-3.0h_Rep22459Shoots N1092AtGen_6-9312_Heatstress-Shoots-3.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9321_Heatstress-Roots-3.0h_Rep12460Roots N1092AtGen_6-9321_Heatstressoots-3.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9322_Heatstress-Roots-3.0h_Rep22461Roots N1092AtGen_6-9322_Heatstressoots-3.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9411_Heatstress(3h)+3hrecovery-Shoots-6.0h_Rep12466Shoots N1092AtGen_6-9411_Heatstress(3h)+3hrecovery-Shoots-6.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9412_Heatstress(3h)+3hrecovery-Shoots-6.0h_Rep22467Shoots N1092AtGen_6-9412_Heatstress(3h)+3hrecovery-Shoots-6.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9421_Heatstress(3h)+3hrecovery-Roots-6.0h_Rep12468Roots N1092AtGen_6-9421_Heatstress(3h)+3hrecoveryoots-6.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9422_Heatstress(3h)+3hrecovery-Roots-6.0h_Rep22469Roots N1092AtGen_6-9422_Heatstress(3h)+3hrecoveryoots-6.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9511_Heatstress(3h)+9hrecovery-Shoots-12.0h_Rep12470Shoots N1092AtGen_6-9511_Heatstress(3h)+9hrecovery-Shoots-12.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9512_Heatstress(3h)+9hrecovery-Shoots-12.0h_Rep22471Shoots N1092AtGen_6-9512_Heatstress(3h)+9hrecovery-Shoots-12.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9521_Heatstress(3h)+9hrecovery-Roots-12.0h_Rep12472Roots N1092AtGen_6-9521_Heatstress(3h)+9hrecoveryoots-12.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9522_Heatstress(3h)+9hrecovery-Roots-12.0h_Rep22473Roots N1092AtGen_6-9522_Heatstress(3h)+9hrecoveryoots-12.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9611_Heatstress(3h)+21hrecovery-Shoots-24.0h_Rep12474Shoots N1092AtGen_6-9611_Heatstress(3h)+21hrecovery-Shoots-24.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9612_Heatstress(3h)+21hrecovery-Shoots-24.0h_Rep22475Shoots N10929612.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9621_Heatstress(3h)+21hrecovery-Roots-24.0h_Rep12476Roots N10929621.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9622_Heatstress(3h)+21hrecovery-Roots-24.0h_Rep22477Roots N10929622.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9711_Heatstress-Shoots-0.25h_Rep12446Shoots N10929711.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9712_Heatstress-Shoots-0.25h_Rep22447Shoots N10929712.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9721_Heatstress-Roots-0.25h_Rep12448Roots N10929721.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9722_Heatstress-Roots-0.25h_Rep22449Roots N10929722.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9811_Heatstress(3h)+1hrecovery-Shoots-4.0h_Rep12462Shoots N10929811.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9812_Heatstress(3h)+1hrecovery-Shoots-4.0h_Rep22463Shoots N10929812.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9821_Heatstress(3h)+1hrecovery-Roots-4.0h_Rep12464Roots N10929821.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring
AtGen_6-9822_Heatstress(3h)+1hrecovery-Roots-4.0h_Rep22465Roots N10929822.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Heat stress (38°C, samples taken at 0.25, 0.5, 1.0, 3.0h of hs and +1, +3, +9, +21h recovery at 25°C),Group Nover/von Koskull-Döring