NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:145
Title:AtGenExpress:Stress Treatments (Wounding stress)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana. The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wild Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-8111_Woundingstress-Shoots-0.5h_Rep12490Shoots N1092AtGen_6-8111_Woundingstress-Shoots-0.5h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8112_Woundingstress-Shoots-0.5h_Rep22491Shoots N1092AtGen_6-8112_Woundingstress-Shoots-0.5h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8124_Woundingstress-Roots-0.5h_Rep12492Roots N1092AtGen_6-8124_Woundingstressoots-0.5h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8126_Woundingstress-Roots-0.5h_Rep22493Roots N1092AtGen_6-8126_Woundingstressoots-0.5h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8211_Woundingstress-Shoots-1.0h_Rep12494Shoots N1092AtGen_6-8211_Woundingstress-Shoots-1.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8214_Woundingstress-Shoots-1.0h_Rep22495Shoots N1092AtGen_6-8214_Woundingstress-Shoots-1.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8224_Woundingstress-Roots-1.0h_Rep12496Roots N1092AtGen_6-8224_Woundingstressoots-1.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8225_Woundingstress-Roots-1.0h_Rep22497Roots N1092AtGen_6-8225_Woundingstressoots-1.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8313_Woundingstress-Shoots-3.0h_Rep12498Shoots N1092AtGen_6-8313_Woundingstress-Shoots-3.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8314_Woundingstress-Shoots-3.0h_Rep22499Shoots N1092AtGen_6-8314_Woundingstress-Shoots-3.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8324_Woundingstress-Roots-3.0h_Rep12500Roots N1092AtGen_6-8324_Woundingstressoots-3.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8325_Woundingstress-Roots-3.0h_Rep22501Roots N1092AtGen_6-8325_Woundingstressoots-3.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8411_Woundingstress-Shoots-6.0h_Rep12502Shoots N1092AtGen_6-8411_Woundingstress-Shoots-6.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8412_Woundingstress-Shoots-6.0h_Rep22503Shoots N1092AtGen_6-8412_Woundingstress-Shoots-6.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8423_Woundingstress-Roots-6.0h_Rep12504Roots N1092AtGen_6-8423_Woundingstressoots-6.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8424_Woundingstress-Roots-6.0h_Rep22505Roots N1092AtGen_6-8424_Woundingstressoots-6.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8511_Woundingstress-Shoots-12.0h_Rep12506Shoots N1092AtGen_6-8511_Woundingstress-Shoots-12.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8512_Woundingstress-Shoots-12.0h_Rep22507Shoots N1092AtGen_6-8512_Woundingstress-Shoots-12.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8524_Woundingstress-Roots-12.0h_Rep12508Roots N1092AtGen_6-8524_Woundingstressoots-12.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8525_Woundingstress-Roots-12.0h_Rep22509Roots N1092AtGen_6-8525_Woundingstressoots-12.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8611_Woundingstress-Shoots-24.0h_Rep12510Shoots N1092AtGen_6-8611_Woundingstress-Shoots-24.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8612_Woundingstress-Shoots-24.0h_Rep22511Shoots N1092AtGen_6-8612_Woundingstress-Shoots-24.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8621_Woundingstress-Roots-24.0h_Rep12512Roots N10928621.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8622_Woundingstress-Roots-24.0h_Rep22513Roots N1092AtGen_6-8622_Woundingstressoots-24.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8712_Woundingstress-Shoots-0.25h_Rep22487Shoots N1092AtGen_6-8712_Woundingstress-Shoots-0.25h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8715_Woundingstress-Shoots-0.25h_Rep12486Shoots N1092AtGen_6-8715_Woundingstress-Shoots-0.25h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8723_Woundingstress-Roots-0.25h_Rep12488Roots N1092AtGen_6-8723_Woundingstressoots-0.25h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter
AtGen_6-8724_Woundingstress-Roots-0.25h_Rep22489Roots N1092AtGen_6-8724_Woundingstressoots-0.25h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Wounding stress (Punctured with pins), Group Harter