NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:144
Title:AtGenExpress:Stress Treatments (UV-B stress)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana. The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wild Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-7111_UV-Bstress-Shoots-0.5h_Rep12394Shoots N10927111.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7112_UV-Bstress-Shoots-0.5h_Rep22395Shoots N10927112.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7121_UV-Bstress-Roots-0.5h_Rep12396Roots N10927121.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7122_UV-Bstress-Roots-0.5h_Rep22397Roots N10927122.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7211_UV-Bstress-Shoots-1.0h_Rep12398Shoots N10927211.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7212_UV-Bstress-Shoots-1.0h_Rep22399Shoots N10927212.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7221_UV-Bstress-Roots-1.0h_Rep12400Roots N10927221.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7222_UV-Bstress-Roots-1.0h_Rep22401Roots N10927222.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7311_UV-Bstress-Shoots-3.0h_Rep12402Shoots N10927311.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7312_UV-Bstress-Shoots-3.0h_Rep22403Shoots N1092AtGen_6-7312_UV-Bstress-Shoots-3.0h_Rep2-R.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7321_UV-Bstress-Roots-3.0h_Rep12404Roots N10927321.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7322_UV-Bstress-Roots-3.0h_Rep22405Roots N10927322.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7411_UV-Bstress-Shoots-6.0h_Rep12406Shoots N10927411.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7412_UV-Bstress-Shoots-6.0h_Rep22407Shoots N10927412.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7421_UV-Bstress-Roots-6.0h_Rep12408Roots N10927421.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7422_UV-Bstress-Roots-6.0h_Rep22409Roots N10927422.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7511_UV-Bstress-Shoots-12.0h_Rep12410Shoots N10927511.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7512_UV-Bstress-Shoots-12.0h_Rep22411Shoots N10927512.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7521_UV-Bstress-Roots-12.0h_Rep12412Roots N10927521.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7522_UV-Bstress-Roots-12.0h_Rep22413Roots N10927522.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7611_UV-Bstress-Shoots-24.0h_Rep12414Shoots N10927611.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7612_UV-Bstress-Shoots-24.0h_Rep22415Shoots N10927612.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7621_UV-Bstress-Roots-24.0h_Rep12416Roots N10927621.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7622_UV-Bstress-Roots-24.0h_Rep22417Roots N10927622.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7711_UV-Bstress-Shoots-0.25h_Rep12390Shoots N10927711.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7712_UV-Bstress-Shoots-0.25h_Rep22391Shoots N10927712.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7721_UV-Bstress-Roots-0.25h_Rep12392Roots N10927721.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter
AtGen_6-7722_UV-Bstress-Roots-0.25h_Rep22393Roots N10927722.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. UV-B stress (15 min. 1.18 W/m2 Philips TL40W/12), Group Harter