NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:143
Title:AtGenExpress:Stress Treatments (Oxidative stress)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana. The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wild Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-6111_Oxidativestress-Shoots-0.5h_Rep12366Shoots N1092AtGen_6-6111_Oxidativestress-Shoots-0.5h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6112_Oxidativestress-Shoots-0.5h_Rep22367Shoots N1092AtGen_6-6112_Oxidativestress-Shoots-0.5h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6122_Oxidativestress-Roots-0.5h_Rep22369Roots N1092AtGen_6-6122_Oxidativestressoots-0.5h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6124_Oxidativestress-Roots-0.5h_Rep12368Roots N1092AtGen_6-6124_Oxidativestressoots-0.5h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6211_Oxidativestress-Shoots-1.0h_Rep12370Shoots N10926211.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6212_Oxidativestress-Shoots-1.0h_Rep22371Shoots N1092AtGen_6-6212_Oxidativestress-Shoots-1.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6223_Oxidativestress-Roots-1.0h_Rep12372Roots N1092AtGen_6-6223_Oxidativestressoots-1.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6224_Oxidativestress-Roots-1.0h_Rep22373Roots N1092AtGen_6-6224_Oxidativestressoots-1.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6311_Oxidativestress-Shoots-3.0h_Rep12374Shoots N1092AtGen_6-6311_Oxidativestress-Shoots-3.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6312_Oxidativestress-Shoots-3.0h_Rep22375Shoots N1092AtGen_6-6312_Oxidativestress-Shoots-3.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6322_Oxidativestress-Roots-3.0h_Rep22377Roots N1092AtGen_6-6322_Oxidativestressoots-3.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6323_Oxidativestress-Roots-3.0h_Rep12376Roots N1092AtGen_6-6323_Oxidativestressoots-3.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6411_Oxidativestress-Shoots-6.0h_Rep12378Shoots N1092AtGen_6-6411_Oxidativestress-Shoots-6.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6412_Oxidativestress-Shoots-6.0h_Rep22379Shoots N1092AtGen_6-6412_Oxidativestress-Shoots-6.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6421_Oxidativestress-Roots-6.0h_Rep12380Roots N1092AtGen_6-6421_Oxidativestressoots-6.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6422_Oxidativestress-Roots-6.0h_Rep22381Roots N1092AtGen_6-6422_Oxidativestressoots-6.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6511_Oxidativestress-Shoots-12.0h_Rep12382Shoots N1092AtGen_6-6511_Oxidativestress-Shoots-12.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6512_Oxidativestress-Shoots-12.0h_Rep22383Shoots N1092AtGen_6-6512_Oxidativestress-Shoots-12.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6523_Oxidativestress-Roots-12.0h_Rep12384Roots N1092AtGen_6-6523_Oxidativestressoots-12.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6524_Oxidativestress-Roots-12.0h_Rep22385Roots N1092AtGen_6-6524_Oxidativestressoots-12.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6611_Oxidativestress-Shoots-24.0h_Rep12386Shoots N1092AtGen_6-6611_Oxidativestress-Shoots-24.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6612_Oxidativestress-Shoots-24.0h_Rep22387Shoots N1092AtGen_6-6612_Oxidativestress-Shoots-24.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6621_Oxidativestress-Roots-24.0h_Rep12388Roots N1092AtGen_6-6621_Oxidativestressoots-24.0h_Rep1.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.
AtGen_6-6625_Oxidativestress-Roots-24.0h_Rep22389Roots N1092AtGen_6-6625_Oxidativestressoots-24.0h_Rep2.cel
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Oxidative stress (10 µM Methyl Viologen), Group Bartels. Methyl Viologen was added to a final concectration of 10µM in the Media. To add the reagent the raft was lifted out A magnetic stir bar and a stirrer were used to mix the media and the added reagent. After the rafts were put back in the boxes, they were transferred back to the climate chamber.