NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Control of lignification
Description:Lignin is a carbon rich polymer that is embedded in the cell walls of specialised plant cells. The metabolic cost of making lignin is very high. Therefore lignin biosynthesis must be carefully regulated so as to balance the demands of growth and development against the availability of resources to make this compound. Little is known about the signalling mechanisms that underpin this regulation. We have devised experiments which dissect the regulation of lignification. The "base-line" for our analyses are wild-type plants that have been grown in liquid medium in the absence of sugars in the dark. These plants develop a vascular system but do not lignify. We compare plants grown in this way against mutants and transgenic plants that are able to lignify even under these conditions. By comparing these mutants and transgenic plants against the wild type we are able to uncover the regulatory mechanisms that control when and where lignins are made. Our analyses would include:1) wild-type dark grown no sugar 7 days post germination - no lignification 2) wild-type dark grown_plus sucrose 7 days post germination - lignification 3) wild-type light grown no sugar 7 days post germination - lignification 4) wild-type 6 days dark 1 day light grown no sugar- lignification 5) de-etiolated 3 mutant dark grown no sugar 7 days post germination - some lignification 6) de-etiolated 3 mutant dark grown_plus sucrose 7 days post germination - extensive ectopic lignification 7) zebra 1 mutant dark grown no sugar 7 days post germination - strong ectopic lignification 8) zebra 2 mutant dark grown no sugar 7 days post germination - strong ectopic lignification 9) de-etiolated 3 mutant _plus co-suppressed MYB61 dark grown_plus sugar 7 days post germination - low lignification - two lines (duplicated) 10) wild-type _plus overexpressed MYB61dark grown no sugar 7 days post germination - ectopic lignification - 3 lines (duplicated) 11) de-etiolated 3 mutant _plus kanamycin dark grown_plus sugar 7 days post germination - wild-type phenotype restored (duplicated) We have already completed this analysis once using Affymetrix GeneRNAs. The results of this analysis have been spectacular and using cluster analysis we have already identified several putative "regulons". In order to validate these data we ask permission to complete a biological replicate of this experiment and to extend our analysis of the transgenic plants to include duplicates of the following: 1) 3 independent lines of MYB61 overexpressing plants 2) 2 independent lines of de-etiolated 3 mutants with MYB61 knocked-out 3) de-etiolated 3 mutant treated with spermidine - restores wild type phenotype This experiment will require 20 RNAs. All plants are in a Col-O background
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Campb-317-Col0-Suc-Rep194Col-0Whole Plant N1092MC001_ATH1_A1-Campb-317.CEL
Campb-318-Col0-Suc-Rep296Col-0Whole Plant N1092MC001_ATH1_A2-Campb-318.CEL
Campb-319-Col0-Suc-Rep398Col-0Whole Plant N1092MC001_ATH1_A3-Campb-319.CEL
Campb-320-Col0-noSuc-Rep1100Col-0Whole Plant N1092MC001_ATH1_A4-Campb-320.CEL
Campb-321-Col0-noSuc-Rep2102Col-0Whole Plant N1092MC001_ATH1_A5-Campb-321.CEL
Campb-322-Col0-noSuc-Rep3104Col-0Whole Plant N1092MC001_ATH1_A6-Campb-322.CEL
Campb-323-MYB61-Suc-Rep195Col-0 (N1092)Whole Plant MC001_ATH1_A7-Campb-323.CEL
Campb-324-MYB61-Suc-Rep297Col-0 (N1092)Whole Plant MC001_ATH1_A8-Campb-324(new).CEL
Campb-325-MYB61-Suc-Rep399Col-0 (N1092)Whole Plant MC001_ATH1_A9-Campb-325.CEL
Campb-326-MYB61-noSuc-Rep1101Col-0 (N1092)Whole Plant MC001_ATH1_A10-Campb-326.CEL
Campb-327-MYB61-noSuc-Rep2103Col-0 (N1092)Whole Plant MC001_ATH1_A11-Campb-327.CEL
Campb-328-MYB61-noSuc-Rep3105Col-0 (N1092)Whole Plant MC001_ATH1_A12-Campb-328(new).CEL
Campb-329-MYB61oe-Suc-Rep1312Col-0 (N1092)Whole Plant MC001_ATH1_A13-Campb-329(new).CEL
Campb-330-MYB61oe-Suc-Rep2313Col-0 (N1092)Whole Plant MC001_ATH1_A14-Campb-330(new).CEL
Campb-331-MYB61oe-Suc-Rep3314Col-0 (N1092)Whole Plant MC001_ATH1_A15-Campb-331.CEL
Campb-332-MYB61oe-noSuc-Rep1315Col-0 (N1092)Whole Plant MC001_ATH1_A16-Campb-332.CEL
Campb-333-MYB61oe-noSuc-Rep2316Col-0 (N1092)Whole Plant MC001_ATH1_A17-Campb-333(new).CEL
Campb-334-MYB61oe-noSuc-Rep3317Col-0 (N1092)Whole Plant MC001_ATH1_A18-Campb-334.CEL
Campb-335-Col0-noSuc-AGA-Rep1318Whole Plant N1092MC001_AG_A19-Campb-335.CEL
Campb-336-det3-noSuc-AGA-Rep1319Whole Plant N6160MC001_AG_A20-Campb-336.CEL
Campb-337-det3-noSuc-AGA-Rep2320Whole Plant N6160MC001_AG_A21-Campb-337.CEL
Campb-338-zeb1-noSuc-AGA-Rep1321Whole Plant MC001_AG_A22-Campb-338.CEL
Campb-339-zeb1-noSuc-AGA-Rep2322Whole Plant MC001_AG_A23-Campb-339.CEL
Campb-340-zeb2-noSuc-AGA-Rep1323Whole Plant MC001_AG_A24-Campb-340.CEL
Campb-341-zeb2-noSuc-AGA-Rep2324Whole Plant MC001_AG_A25-Campb-341.CEL
Campb-342-Col0-noSuc-AGA-Rep2325Whole Plant N1092MC001_AG_A26-Campb-342.CEL
Campb-343-Col0-Suc-AGA-Rep1326Whole Plant N1092MC001_AG_A27-Campb-343.CEL
Campb-344-Col0-Suc-AGA-Rep2392Whole Plant N1092MC001_AG_A28-Campb-344.CEL
Campb-Col0-noSuc-AGA-Rep31053Whole Plant N1092arabidopsis 1 28.11.CEL
Campb-Col0-Suc-AGA-Rep31057Whole Plant N1092arabidopsis 8 28.11.CEL
Campb-det3-noSuc-AGA-Rep31054Whole Plant N6160arabidopsis 2 28.11.CEL
Campb-zeb1-noSuc-AGA-Rep31055Whole Plant arabidopsis 5 28.11.CEL
Campb-zeb2-noSuc-AGA-Rep31056Whole Plant arabidopsis 6 28.11.CEL