NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:137
Title:AtGenExpress:Stress Treatments (Control plants)
Date:2004-10-01
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Seeds of Arabidopsis thaliana Wild Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.AtGenExpress: Stress treatmentsSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media. After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media. At day 16 stress treatment started at 3 hrs of light period; samples taken at 0.5, 1, 3, 6, 12, 24 h after treatment (in selected indicated cases 0.25h and 4.0h too), control samples include 0h; roots and shoots were prepared separately; all treatments and preparations were done on the same batch of seedlings in one place (Lab J.Kudla/Ulm, Germany) by coworkers from the indicated groups.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_6-0011_Control-Shoots-0h_Rep12206Shoots N10920011.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0012_Control-Shoots-0h_Rep22207Shoots N10920012.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0021_Control-Roots-0h_Rep12208Roots N10920021.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0022_Control-Roots-0h_Rep22209Roots N10920022.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0111_Control-Shoots-0.5h_Rep12214Shoots N10920111.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0112_Control-Shoots-0.5h_Rep22215Shoots N10920112.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0121_Control-Roots-0.5h_Rep12216Roots N10920121.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0122_Control-Roots-0.5h_Rep22217Roots N10920122.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0211_Control-Shoots-1.0h_Rep12218Shoots N10920211.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0212_Control-Shoots-1.0h_Rep22219Shoots N10920212.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0221_Control-Roots-1.0h_Rep12220Roots N10920221.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0222_Control-Roots-1.0h_Rep22221Roots N10920222.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0311_Control-Shoots-3.0h_Rep12222Shoots N10920311.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0312_Control-Shoots-3.0h_Rep22223Shoots N10920312.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0321_Control-Roots-3.0h_Rep12224Roots N10920321.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0322_Control-Roots-3.0h_Rep22225Roots N10920322.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0411_Control-Shoots-6.0h_Rep12230Shoots N10920411.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0412_Control-Shoots-6.0h_Rep22231Shoots N10920412.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0421_Control-Roots-6.0h_Rep12232Roots N10920421.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0422_Control-Roots-6.0h_Rep22233Roots N10920422.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0511_Control-Shoots-12.0h_Rep12234Shoots N10920511.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0512_Control-Shoots-12.0h_Rep22235Shoots N10920512.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0521_Control-Roots-12.0h_Rep12236Roots N10920521.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0522_Control-Roots-12.0h_Rep22237Roots N10920522.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0611_Control-Shoots-24.0h_Rep12238Shoots N10920611.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0612_Control-Shoots-24.0h_Rep22239Shoots N10920612.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0621_Control-Roots-24.0h_Rep12240Roots N10920621.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0622_Control-Roots-24.0h_Rep22241Roots N10920622.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0711_Control-Shoots-0.25h_Rep12210Shoots N10920711.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0712_Control-Shoots-0.25h_Rep22211Shoots N10920712.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0721_Control-Roots-0.25h_Rep12212Roots N10920721.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0722_Control-Roots-0.25h_Rep22213Roots N10920722.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0811_Control-Shoots-4.0h_Rep12226Shoots N10920811.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0812_Control-Shoots-4.0h_Rep22227Shoots N10920812.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0821_Control-Roots-4.0h_Rep12228Roots N10920821.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.
AtGen_6-0822_Control-Roots-4.0h_Rep22229Roots N10920822.CEL
Treatment: At day 16, stress treatment started at 3 hours of light treatment. Control plants, Group Kudla. The plants were treated like the treated plants; e.g.: Transfer of Magenta boxes out of the climate chamber. Opening of the boxes and lifting the raft as long as the treatments last. Then boxes were transferred back to the climate chamber.