NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:136
Title:Expression patterns of genes induced by sugar accumulation during early leaf senescence
Date:2005-04-04
Description:The aim of our experiment is to analyse the expression pattern of genes induced by sugar accumulation during early leaf senescence.We have recently shown that senescence is induced by glucose in combination with low, but not with high nitrogen supply (Wingler et al. (2004), New Phytol, in press).We would like to use Affymetrix analysis to compare senescence-specific gene expression in plants grown on (i) low nitrogen without glucose (LNM), (ii) low nitrogen with 2% glucose (LNG) and (iii) high nitrogen with 2% glucose (HNG). Comparison of (i) and (ii) will allow us to determine the effect of glucose, while comparison of (ii) and (iii) will reveal effects of nitrogen supply.For this experiment, plants will be harvested on day 30, when first signs of senescence become visible on the LNG medium. All samples will be taken around mid-day. For each sample, 12 plants from 3 different petri dishes will be pooled. We are planning to analyse 3 independent samples per treatment, i.e. 9 samples in total. RNA will be extracted from whole leaf rosettes (without roots and inflorescences) using the RNeasy Plant Kit (Qiagen).At a later stage, we are also intending to compare gene expression in wild-type plants with expression in the abi5-1 mutant (Ws-2 background), which shows a delay in sugar-induced senescence (Wingler, Astrid, Marès, Magali & Pourtau, Nathalie (2004)Spatial patterns and metabolic regulation of photosynthetic parameters during leaf senescence.New Phytologist 161 (3), 781-789). We have therefore chosen to use Ws-2 for the experiment proposed here. The experiment with abi5-1 will be conducted once we have obtained data on treatment-specific effects in Ws-2.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Pourtau_1-1_lowN_Rep1_ATH11649whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-1_lowN_Rep1_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only).
Pourtau_1-2_lowN_Rep2_ATH11646whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-2_lowN_Rep2_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only).
Pourtau_1-3_lowN_Rep3_ATH11643whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-3_lowN_Rep3_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only).
Pourtau_1-4_lowN-glu_Rep1_ATH11648whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-4_lowN-glu_Rep1_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). For sugar treatments, 2% of glucose will be added to the corresponding medium.
Pourtau_1-5_lowN-glu_Rep2_ATH11645whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-5_lowN-glu_Rep2_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). For sugar treatments, 2% of glucose will be added to the corresponding medium.
Pourtau_1-6_lowN-glu_Rep3_ATH11642whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-6_lowN-glu_Rep3_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 4.7mM (NO3- only). For sugar treatments, 2% of glucose will be added to the corresponding medium.
Pourtau_1-7_highN-glu_Rep1_ATH11647whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-7_highN-glu_Rep1_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 30 mM nitrogen (10.3mM NH4+ and 19.7mM NO3-). For sugar treatments, 2% of glucose will be added to the corresponding medium.
Pourtau_1-8_highN-glu_Rep2_ATH11644whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-8_highN-glu_Rep2_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 30 mM nitrogen (10.3mM NH4+ and 19.7mM NO3-). For sugar treatments, 2% of glucose will be added to the corresponding medium.
Pourtau_1-9_highN-glu_Rep3_ATH11641whole leaf rosettes without the roots and inflorescences N1601Pourtau_1-9_highN-glu_Rep3_ATH1.CEL
Treatment: Half-strength Murashige and Skoog medium containing 30 mM nitrogen (10.3mM NH4+ and 19.7mM NO3-). For sugar treatments, 2% of glucose will be added to the corresponding medium.