NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 127 |
| Title: | Comparative transcriptome analysis of wild type and two knockout alleles of At-CAMTA |
| Date: | 2004-11-19 |
| Description: | Calcium has been shown to be an important signalling molecule in the transduction of abiotic stress signals in Arabidopsis thaliana. Alteration of the calcium signature through the use of inhibitors has been shown to result in changes in the expression of certain downstream abiotic stress-induced genes. However, the communication of this signal through specific calcium-sensitive intermediate molecules has remained poorly elucidated. Various candidate molecules exist and including calcinurin, calcium-dependent protein kinases and calmodulin. Recently, a screen of an Arabidopsis thaliana cDNA expression library with calmodulin resulted in the isolation of the protein designated calmodulin-binding transcriptional activator or AtCAMTA that may function as one of these calcium-sensitive intermediate molecules. The Arabidopsis genome contains six CAMTA genes (AtCAMTA1-6), all of which are expressed in varying tissues throughout development. AtCAMTA1 was shown to function as a transcriptional activator through the expression of a chimeric protein consisting of the bacteria LexA protein fused to various segments of AtCAMTA expressed in yeast. In order to identify putative target genes of AtCAMTA1 in Arabidopsis we plan to analyse the transcriptome of AtCAMTA1 loss-of-function mutants. The gene expression pattern of two previously characterised alleles of AtCAMTA1 will be compared to wild type. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Scrase-Field_1-1_Col-0-wildtype_Rep1_ATH1 | 1586 | Cell culture experiment | Scras-Field_1-1_CWT_REP1_ATH1.CEL | ||
| Scrase-Field_1-2_camta1-2-Col-0_Rep1_ATH1 | 1587 | Col-0 | Whole seedling | Scras-Field_1-2_CK_REP1_ATH1.CEL | |
| Scrase-Field_1-3_Col-0-wildtype_Rep2_ATH1 | 1588 | Cell culture experiment | Scras-Field_1-3_CWT_REP2_ATH1.CEL | ||
| Scrase-Field_1-4_camta1-2-Col-0_Rep2_ATH1 | 1589 | Col-0 | Whole seedling | Scras-Field_1-4_CK_REP2_ATH1.CEL |