NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:124
Title:AtGenExpress:Light treatments
Date:2004-07-09
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana (Hybridisations done at NASC). The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Series 1: Growth conditions: Sterilized seeds will be stratified at 4°C for 3 days, exposed to white light for 2 h to induce germination, and grown on MS agar plates (0.9 % agar) without sugar in total darkness for 4 days at 22°C. Seedlings will then be transferred to the light conditions described for each slide below for 1 h (to identify early induced genes) and 4 hrs (maximum expression of the first initial light response of most target genes), respectively. All light treatments will be done in parallel to minimize the number of dark controls. All samples will be done in triplicate and only with shoots.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_D-10_1-FS_REP1_ATH11508mainly hypocotyl and cotyledons N1092AtGen_D-10_1-FS_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 45 min continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-11_1-PS_REP1_ATH11509mainly hypocotyl and cotyledons N1092AtGen_D-11_1-PS_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 44 min of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-12_1-RS_REP1_ATH11510mainly hypocotyl and cotyledons N1092AtGen_D-12_1-RS_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 45 min continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-13_1-BS_REP1_ATH11511mainly hypocotyl and cotyledons N1092AtGen_D-13_1-BS_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 45 min continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-14_1-AS_REP1_ATH11512mainly hypocotyl and cotyledons N1092AtGen_D-14_1-AS_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 40 min of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338
AtGen_D-15_1-US_REP1_ATH11513mainly hypocotyl and cotyledons N1092AtGen_D-15_1-US_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / Sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol -> pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 40 min of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338
AtGen_D-16_1-WS_REP1_ATH11514mainly hypocotyl and cotyledons N1092AtGen_D-16_1-WS_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 45 min continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-17_2-DL_REP2_ATH11560mainly hypocotyl and cotyledons N1092AtGen_D-17_2-DL_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 4h of complete darkness (dark control)
AtGen_D-18_2-FL_REP2_ATH11515mainly hypocotyl and cotyledons N1092AtGen_D-18_2-FL_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 4 h continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-19_2-PL_REP2_ATH11516mainly hypocotyl and cotyledons N1092AtGen_D-19_2-PL_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 4 h of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-1_1-DL_REP1_ATH11499mainly hypocotyl and cotyledons N1092AtGen_D-1_1-DL_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol -> pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: 4h of complete darkness (dark control)
AtGen_D-20_2-RL_REP2_ATH11517mainly hypocotyl and cotyledons N1092AtGen_D-20_2-RL_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 4 h continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-21_2-BL_REP2_ATH11518mainly hypocotyl and cotyledons N1092AtGen_D-21_2-BL_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 4 h continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-22_2-AL_REP2_ATH11519mainly hypocotyl and cotyledons N1092AtGen_D-22_2-AL_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 4 h of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338
AtGen_D-23_2-UL_REP2_ATH11520mainly hypocotyl and cotyledons N1092AtGen_D-23_2-UL_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 4 h of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338
AtGen_D-24_2-WL_REP2_ATH11521mainly hypocotyl and cotyledons N1092AtGen_D-24_2-WL_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 4 h continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-25_2-DS_REP2_ATH11522mainly hypocotyl and cotyledons N1092AtGen_D-25_2-DS_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: 45 min of complete darkness (dark control)
AtGen_D-26_1-FS_REP2_ATH11561mainly hypocotyl and cotyledons N1092AtGen_D-26_2-FS_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: continuous far-red light / time course: 45 min continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-27_2-PS_REP2_ATH11524mainly hypocotyl and cotyledons N1092AtGen_D-27_2-PS_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 44 min of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-28_2-RS_REP2_ATH11525mainly hypocotyl and cotyledons N1092AtGen_D-28_2-RS_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 45 min continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-29_2-BS_REP2_ATH11526mainly hypocotyl and cotyledons N1092AtGen_D-29_2-BS_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 45 min continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-2_1-FL_REP1_ATH11500mainly hypocotyl and cotyledons N1092AtGen_D-2_1-FL_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 4 h continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-30_2-AS_REP2_ATH11527mainly hypocotyl and cotyledons N1092AtGen_D-30_2-AS_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 40 min of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338
AtGen_D-31_2-US_REP2_ATH11528mainly hypocotyl and cotyledons N1092AtGen_D-31_2-US_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / Sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol -> pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 40 min of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338
AtGen_D-32_2-WS_REP2_ATH11529mainly hypocotyl and cotyledons N1092AtGen_D-32_2-WS_REP2_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 45 min continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-33_3-DL_REP3_ATH11531mainly hypocotyl and cotyledons N1092AtGen_D-33_3-DL_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: 4h of complete darkness (dark control)
AtGen_D-34_3-FL_REP3_ATH11533mainly hypocotyl and cotyledons N1092AtGen_D-34_3-FL_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 4 h continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-35_3-PL_REP3_ATH11535mainly hypocotyl and cotyledons N1092AtGen_D-35_3-PL_REP3_ATH1.CEL
Treatment: Treatment before light irradiation Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 4 h of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-36_3-RL_REP3_ATH11537mainly hypocotyl and cotyledons N1092AtGen_D-36_3-RL_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 4 h continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-37_3-BL_REP3_ATH11539mainly hypocotyl and cotyledons N1092AtGen_D-37_3-BL_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 4 h continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-38_3-AL_REP3_ATH11541mainly hypocotyl and cotyledons N1092AtGen_D-38_3-AL_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 4 h of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338
AtGen_D-39_3-UL_REP3_ATH11543mainly hypocotyl and cotyledons N1092AtGen_D-39_3-UL_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 4 h of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338
AtGen_D-3_1-PL_REP1_ATH11501mainly hypocotyl and cotyledons N1092AtGen_D-3_1-PL_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 4 h of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-40_3-WL_REP3_ATH11544mainly hypocotyl and cotyledons N1092AtGen_D-40_3-WL_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 4 h continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-41_3-DS_REP3_ATH11545mainly hypocotyl and cotyledons N1092AtGen_D-41_3-DS_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: 45 min of complete darkness (dark control)
AtGen_D-42_3-FS_REP3_ATH11547mainly hypocotyl and cotyledons N1092AtGen_D-42_3-FS_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 45 min continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-43_3-PS_REP3_ATH11549mainly hypocotyl and cotyledons N1092AtGen_D-43_3-PS_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 44 min of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-44_3-RS_REP3_ATH11551mainly hypocotyl and cotyledons N1092AtGen_D-44_3-RS_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 45 min continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-45_3-BS_REP3_ATH11553mainly hypocotyl and cotyledons N1092AtGen_D-45_3-BS_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 45 min continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-46_3-AS_REP3_ATH11555mainly hypocotyl and cotyledons N1092AtGen_D-46_3-AS_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 40 min of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338
AtGen_D-47_3-US_REP3_ATH11557mainly hypocotyl and cotyledons N1092AtGen_D-47_3-US_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / Sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol -> pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 40 min of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338
AtGen_D-48_3-WS_REP3_ATH11559mainly hypocotyl and cotyledons N1092AtGen_D-48_3-WS_REP3_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 45 min continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-4_1-RL_REP1_ATH11502mainly hypocotyl and cotyledons N1092AtGen_D-4_1-RL_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 4 h continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-5_1-BL_REP1_ATH11503mainly hypocotyl and cotyledons N1092AtGen_D-5_1-BL_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 4 h continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-6_1-AL_REP1_ATH11504mainly hypocotyl and cotyledons N1092AtGen_D-6_1-AL_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 4 h of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338
AtGen_D-7_1-UL_REP1_ATH11505mainly hypocotyl and cotyledons N1092AtGen_D-7_1-UL_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 4 h of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338
AtGen_D-8_1-WL_REP1_ATH11506mainly hypocotyl and cotyledons N1092AtGen_D-8_1-WL_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: continuous white light / time course: 4 h continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C
AtGen_D-9_1-DS_REP1_ATH11507mainly hypocotyl and cotyledons N1092AtGen_D-9_1-DS_REP1_ATH1.CEL
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 45 min of complete darkness (dark control)