Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
AtGen_D-10_1-FS_REP1_ATH1 | 1508 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-10_1-FS_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 45 min continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-11_1-PS_REP1_ATH1 | 1509 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-11_1-PS_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 44 min of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-12_1-RS_REP1_ATH1 | 1510 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-12_1-RS_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 45 min continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-13_1-BS_REP1_ATH1 | 1511 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-13_1-BS_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 45 min continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-14_1-AS_REP1_ATH1 | 1512 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-14_1-AS_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 40 min of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338 |
AtGen_D-15_1-US_REP1_ATH1 | 1513 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-15_1-US_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / Sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol -> pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 40 min of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338 |
AtGen_D-16_1-WS_REP1_ATH1 | 1514 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-16_1-WS_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 45 min continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-17_2-DL_REP2_ATH1 | 1560 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-17_2-DL_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 4h of complete darkness (dark control) |
AtGen_D-18_2-FL_REP2_ATH1 | 1515 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-18_2-FL_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 4 h continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-19_2-PL_REP2_ATH1 | 1516 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-19_2-PL_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 4 h of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-1_1-DL_REP1_ATH1 | 1499 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-1_1-DL_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol -> pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: 4h of complete darkness (dark control) |
AtGen_D-20_2-RL_REP2_ATH1 | 1517 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-20_2-RL_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 4 h continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-21_2-BL_REP2_ATH1 | 1518 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-21_2-BL_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 4 h continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-22_2-AL_REP2_ATH1 | 1519 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-22_2-AL_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 4 h of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338 |
AtGen_D-23_2-UL_REP2_ATH1 | 1520 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-23_2-UL_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 4 h of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338 |
AtGen_D-24_2-WL_REP2_ATH1 | 1521 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-24_2-WL_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 4 h continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-25_2-DS_REP2_ATH1 | 1522 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-25_2-DS_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: 45 min of complete darkness (dark control) |
AtGen_D-26_1-FS_REP2_ATH1 | 1561 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-26_2-FS_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: continuous far-red light / time course: 45 min continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-27_2-PS_REP2_ATH1 | 1524 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-27_2-PS_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 44 min of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-28_2-RS_REP2_ATH1 | 1525 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-28_2-RS_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 45 min continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-29_2-BS_REP2_ATH1 | 1526 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-29_2-BS_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 45 min continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-2_1-FL_REP1_ATH1 | 1500 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-2_1-FL_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 4 h continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-30_2-AS_REP2_ATH1 | 1527 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-30_2-AS_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 40 min of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338 |
AtGen_D-31_2-US_REP2_ATH1 | 1528 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-31_2-US_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / Sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol -> pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 40 min of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338 |
AtGen_D-32_2-WS_REP2_ATH1 | 1529 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-32_2-WS_REP2_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 45 min continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-33_3-DL_REP3_ATH1 | 1531 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-33_3-DL_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: 4h of complete darkness (dark control) |
AtGen_D-34_3-FL_REP3_ATH1 | 1533 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-34_3-FL_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 4 h continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-35_3-PL_REP3_ATH1 | 1535 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-35_3-PL_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 4 h of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-36_3-RL_REP3_ATH1 | 1537 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-36_3-RL_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 4 h continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-37_3-BL_REP3_ATH1 | 1539 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-37_3-BL_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 4 h continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-38_3-AL_REP3_ATH1 | 1541 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-38_3-AL_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 4 h of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338 |
AtGen_D-39_3-UL_REP3_ATH1 | 1543 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-39_3-UL_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 4 h of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338 |
AtGen_D-3_1-PL_REP1_ATH1 | 1501 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-3_1-PL_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 4 h of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-40_3-WL_REP3_ATH1 | 1544 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-40_3-WL_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 4 h continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-41_3-DS_REP3_ATH1 | 1545 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-41_3-DS_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: 45 min of complete darkness (dark control) |
AtGen_D-42_3-FS_REP3_ATH1 | 1547 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-42_3-FS_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous far-red light / time course: 45 min continuous far-red light / light intensity: 10 µmol m-2 s-1 / filter: 715 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 715 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-43_3-PS_REP3_ATH1 | 1549 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-43_3-PS_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: red light pulse / time course: 1 min red light pulse followed by 44 min of darkness / light intensity: 50 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-44_3-RS_REP3_ATH1 | 1551 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-44_3-RS_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 45 min continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-45_3-BS_REP3_ATH1 | 1553 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-45_3-BS_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 45 min continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-46_3-AS_REP3_ATH1 | 1555 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-46_3-AS_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 40 min of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338 |
AtGen_D-47_3-US_REP3_ATH1 | 1557 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-47_3-US_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / Sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol -> pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 40 min of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338 |
AtGen_D-48_3-WS_REP3_ATH1 | 1559 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-48_3-WS_REP3_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous white light / time course: 45 min continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-4_1-RL_REP1_ATH1 | 1502 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-4_1-RL_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: light treatment: continuous red light / time course: 4 h continuous red light / light intensity: 10 µmol m-2 s-1 / filter: KG65 double glas filter (Balzers, Liechtenstein), lamba(max) = 650 nm, half-bandwidth = 15 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-5_1-BL_REP1_ATH1 | 1503 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-5_1-BL_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation Light treatment: continuous blue light / time course: 4 h continuous blue light / light intensity: 10 µmol m-2 s-1 / filter: 453 nm DAL interference filter (Schott, Mainz, Germany), lamba(max) = 453 nm, half-bandwidth = 18 nm / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-6_1-AL_REP1_ATH1 | 1504 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-6_1-AL_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: UV-A light pulse / time course: 5 min UV-A light pulse followed by 4 h of darkness / light intensity: 7 W m-2 / filter: WG327 quarz filter, 3 mm, (Schott, Mainz, Germany), cut-off filter with half-maximal transmission at 327 nm / light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm / Temperature: 22°C / literature: PMID 14739338 |
AtGen_D-7_1-UL_REP1_ATH1 | 1505 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-7_1-UL_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 5 min UV-A/B light pulse followed by 4 h of darkness. Light intensity: 7 W m-2. filter: WG307 quarz filter, 3 mm, (Schott, Mainz, Germany),Cut-off filter with half-maximal transmission at 307 nm. Light source: 6 x Philips TL 40W/12 UV fluorescent tubes, lamba(max) = 310 nm, half-bandwidth = 40 nm. Temperature: 22C. PMID 14739338 |
AtGen_D-8_1-WL_REP1_ATH1 | 1506 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-8_1-WL_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: continuous white light / time course: 4 h continuous white light / light intensity: 10 µmol m-2 s-1 / filter: 10 % neutral glas filter (Balzers, Liechtenstein) / light source: Leitz Prado 500-W universal projector (Leitz, Wetzlar, Germany) together with Osram Xenophot longlife lamps (Osram, Berlin, Germany) / Temperature: 22°C |
AtGen_D-9_1-DS_REP1_ATH1 | 1507 | | mainly hypocotyl and cotyledons | N1092 | AtGen_D-9_1-DS_REP1_ATH1.CEL |
Treatment: Treatment before light irradiation: Amount of dry seeds: 18 mg / sterilisation: 3 min 70 % Ethanol, stir with over-head tumbler -> short centrifugation ->resuspend in 100 % Ethanol ->pipette on sterile filter paper ->air dry in a sterile bench / Growth media: MS agar (1.2 %) covered with a sterile filter paper (Schleicher and Schuell, Rundfilter 595, Dassel, Germany) in petridishes / Stratification: 8° C, 48 h / Germination induction: 2 h red light / Growth: 94 h in complete darkness at 22°C before onset of irradiation. Light treatment: 45 min of complete darkness (dark control) |