NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:123
Title:AtGenExpress:Response to Phytophthora infestans
Date:2004-07-09
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana (Hybridisations done at NASC)The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Series 2: Base line experiment for pathogen infection. Growth and infection conditions: Seeds will be stratified for 3 days at 4°C and sown on soil. Plants are grown at 22°C under a 8/16 hour light/dark regime in Percival growth chambers. All pathogen treatments will be performed on leaves of 5-weeks old plants. Bacterial infiltrations will be performed with 10-8 cfu/ml in 10 mM MgCl2. 5x105 spores of Phytophthora infestans in water will be applied to leaf surfaces. LPS (100 µg/ml), HrpZ (1 µM), NPP1 (2 µM) or flagellin (1 µM flg22) will be applied in water. Experiments should be performed in triplicate at the time points indicated below.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_C-10_1-Pi-6_REP1_ATH11490leaves AtGen_C-10_1-Pi-6_REP1_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 6 hours post-treatment.
AtGen_C-11_2-Pi-6_REP2_ATH11491leaves AtGen_C-11_2-Pi-6_REP2_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 6 hours post-treatment.
AtGen_C-12_3-Pi-6_REP3_ATH11492leaves AtGen_C-12_3-Pi-6_REP3_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 6 hours post-treatment.
AtGen_C-13_1-Pi-12_REP1_ATH11493leaves AtGen_C-13_1-Pi-12_REP1_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 12 hours post-treatment.
AtGen_C-14_2-Pi-12_REP2_ATH11494leaves AtGen_C-14_2-Pi-12_REP2_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 12 hours post-treatment.
AtGen_C-15_3-Pi-12_REP3_ATH11495leaves AtGen_C-15_3-Pi-12_REP3_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 12 hours post-treatment.
AtGen_C-16_1-Pi-24_REP1_ATH11496leaves AtGen_C-16_1-Pi-24_REP1_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 24 hours post-treatment.
AtGen_C-17_2-Pi-24_REP2_ATH11497leaves AtGen_C-17_2-Pi-24_REP2_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 24 hours post-treatment.
AtGen_C-18_3-Pi-24_REP3_ATH11498leaves AtGen_C-18_3-Pi-24_REP3_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Phytophthora infestans drops (10 000 spores/drop) on leaves as possible. Trays covered with lid after treatment. Leaves harvested 24 hours post-treatment.
AtGen_C-1_1-C-6_REP1_ATH11481leaves AtGen_C-1_1-C-6_REP1_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 6 hours post-treatment.
AtGen_C-2_2-C-6_REP2_ATH11482leaves AtGen_C-2_2-C-6_REP2_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 6 hours post-treatment.
AtGen_C-3_4-C-6_REP3_ATH11483leaves AtGen_C-3_4-C-6_REP3_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 6 hours post-treatment.
AtGen_C-4_1-C-12_REP1_ATH11484leaves AtGen_C-4_1-C-12_REP1_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 12 hours post-treatment.
AtGen_C-5_2-C-12_REP2_ATH11485leaves AtGen_C-5_2-C-12_REP2_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 12 hours post-treatment.
AtGen_C-6_3-C-12_REP3_ATH11486leaves AtGen_C-6_3-C-12_REP3_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 12 hours post-treatment.
AtGen_C-7_1-C-24_REP1_ATH11487leaves AtGen_C-7_1-C-24_REP1_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 24 hours post-treatment.
AtGen_C-8_2-C-24_REP2_ATH11488leaves AtGen_C-8_2-C-24_REP2_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 24 hours post-treatment.
AtGen_C-9_3-C-24_REP3_ATH11489leaves AtGen_C-9_3-C-24_REP3_ATH1.CEL
Treatment: Plants were moved to growth chamber with 20°C day / 18°C night for treatment. As many Water drops on leaves as possible. Trays covered with lid after treatment. Leaves harvested 24 hours post-treatment.