NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:122
Title:AtGenExpress:Response to bacterial-(LPS, HrpZ, Flg22) and oomycete-(NPP1) derived elicitors
Date:2004-07-09
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana (Hybridisations done at NASC)The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Series 2: Base line experiment for pathogen infection. Growth and infection conditions: Seeds will be stratified for 3 days at 4°C and sown on soil. Plants are grown at 22°C under a 8/16 hour light/dark regime in Percival growth chambers. All pathogen treatments will be performed on leaves of 5-weeks old plants. Bacterial infiltrations will be performed with 10-8 cfu/ml in 10 mM MgCl2. 5x105 spores of Phytophthora infestans in water will be applied to leaf surfaces. LPS (100 µg/ml), HrpZ (1 µM), NPP1 (2 µM) or flagellin (1 µM flg22) will be applied in water. Experiments should be performed in triplicate at the time points indicated below.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_B-10_1-3-4_REP1_ATH11448leaves AtGen_B-10_1-3-4_REP1_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST, harvested 4 hours later.
AtGen_B-11_1-4-4_REP1_ATH11449leaves AtGen_B-11_1-4-4_REP1_ATH1.CEL
Treatment: leaves infiltrated with 10 µM HrpZ, harvested 4 hours later.
AtGen_B-12_1-5-4_REP1_ATH11450leaves AtGen_B-12_1-5-4_REP1_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST-NPP1, harvested 4 hours later.
AtGen_B-13_1-6-4_REP1_ATH11451leaves AtGen_B-13_1-6-4_REP1_ATH1.CEL
Treatment: leaves infiltrated with 1 µM Flg-22, harvested 4 hours later.
AtGen_B-14_1-7-4_REP1_ATH11452leaves AtGen_B-14_1-7-4_REP1_ATH1.CEL
Treatment: leaves infiltrated with 100µg/ml LPS from P.syringae pv.tomato DC3000, harvested 4 hours later
AtGen_B-15_2-1-1_REP2_ATH11453leaves AtGen_B-15_2-1-1_REP2_ATH1.CEL
Treatment: leaves infiltrated with water, harvested 1 hour later.
AtGen_B-16_2-2-1_REP2_ATH11454leaves AtGen_B-16_2-2-1_REP2_ATH1.CEL
Treatment: leaves infiltrated with 1 mM CaCl2 + 2.5 mM MgCl2, harvested 1 hour later.
AtGen_B-17_2-3-1_REP2_ATH11455leaves AtGen_B-17_2-3-1_REP2_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST, harvested 1 hour later
AtGen_B-18_2-4-1_REP2_ATH11456leaves AtGen_B-18_2-4-1_REP2_ATH1.CEL
Treatment: leaves infiltrated with 10 µM HrpZ, harvested 1 hour later
AtGen_B-19_2-5-1_REP2_ATH11457leaves AtGen_B-19_2-5-1_REP2_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST-NPP1, harvested 1 hour later.
AtGen_B-1_1-1-1_REP_1_ATH11439leaves AtGen_B-1_1-1-1_REP1_ATH1.CEL
Treatment: leaves infiltrated with water, harvested 1 hour later.
AtGen_B-20_2-6-1_REP2_ATH11458leaves AtGen_B-20_2-6-1_REP2_ATH1.CEL
Treatment: leaves infiltrated with 1 µM Flg-22, harvested 1 hour later.
AtGen_B-21_2-7-1_REP2_ATH11459leaves AtGen_B-21_2-7-1_REP2_ATH1.CEL
Treatment: leaves infiltrated with 100µg/ml LPS from P.syringae pv.tomato DC3000, harvested 1 hour later.
AtGen_B-22_2-1-4_REP2_ATH11460leaves AtGen_B-22_2-1-4_REP2_ATH1.CEL
Treatment: leaves infiltrated with water, harvested 4 hours later.
AtGen_B-23_2-2-4_REP2_ATH11461leaves AtGen_B-23_2-2-4_REP2_ATH1.CEL
Treatment: leaves infiltrated with 1 mM CaCl2 + 2.5 mM MgCl2, harvested 4 hours later.
AtGen_B-24_2-3-4_REP2_ATH11462leaves AtGen_B-24_2-3-4_REP2_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST, harvested 4 hours later.
AtGen_B-25_2-4-4_REP2_ATH11463leaves AtGen_B-25_2-4-4_REP2_ATH1.CEL
Treatment: leaves infiltrated with 10 µM HrpZ, harvested 4 hours later.
AtGen_B-26_2-5-4_REP2_ATH11464leaves AtGen_B-26_2-5-4_REP2_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST-NPP1, harvested 4 hours later.
AtGen_B-27_2-6-4_REP2_ATH11465leaves AtGen_B-27_2-6-4_REP2_ATH1.CEL
Treatment: leaves infiltrated with 1 µM Flg-22, harvested 4 hours later.
AtGen_B-28_2-7-4_REP2_ATH11466leaves AtGen_B-28_2-7-4_REP2_ATH1.CEL
Treatment: leaves infiltrated with 100µg/ml LPS from P.syringae pv.tomato DC3000, harvested 4 hours later
AtGen_B-29_3-1-1_REP3_ATH11467leaves AtGen_B-29_3-1-1_REP3_ATH1.CEL
Treatment: leaves infiltrated with water, harvested 1 hour later.
AtGen_B-2_1-2-1_REP_1_ATH11440leaves AtGen_B-2_1-2-1_REP1_ATH1.CEL
Treatment: leaves infiltrated with 1 mM CaCl2 + 2.5 mM MgCL2, harvested 1 hour later.
AtGen_B-30_3-2-1_REP3_ATH11468leaves AtGen_B-30_3-2-1_REP3_ATH1.CEL
Treatment: leaves infiltrated with 1 mM CaCl2 + 2.5 mM MgCl2, harvested 1 hour later.
AtGen_B-31_3-3-1_REP3_ATH11469leaves AtGen_B-31_3-3-1_REP3_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST, harvested 1 hour later
AtGen_B-32_3-4-1_REP3_ATH11470leaves AtGen_B-32_3-4-1_REP3_ATH1.CEL
Treatment: leaves infiltrated with 10 µM HrpZ, harvested 1 hour later
AtGen_B-33_3-5-1_REP3_ATH11471leaves AtGen_B-33_3-5-1_REP3_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST-NPP1, harvested 1 hour later.
AtGen_B-34_3-6-1_REP3_ATH11472leaves AtGen_B-34_3-6-1_REP3_ATH1.CEL
Treatment: leaves infiltrated with 1 µM Flg-22, harvested 1 hour later.
AtGen_B-35_3-7-1_REP3_ATH11473leaves AtGen_B-35_3-7-1_REP3_ATH1.CEL
Treatment: leaves infiltrated with 100µg/ml LPS from P.syringae pv.tomato DC3000, harvested 1 hour later.
AtGen_B-36_3-1-4_REP3_ATH11474leaves AtGen_B-36_3-1-4_REP3_ATH1.CEL
Treatment: leaves infiltrated with water, harvested 4 hours later.
AtGen_B-37_3-2-4_REP3_ATH11475leaves AtGen_B-37_3-2-4_REP3_ATH1.CEL
Treatment: leaves infiltrated with 1 mM CaCl2 + 2.5 mM MgCl2, harvested 4 hours later.
AtGen_B-38_3-3-4_REP3_ATH11476leaves AtGen_B-38_3-3-4_REP3_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST, harvested 4 hours later.
AtGen_B-39_3-4-4_REP3_ATH11477leaves AtGen_B-39_3-4-4_REP3_ATH1.CEL
Treatment: leaves infiltrated with 10 µM HrpZ, harvested 4 hours later.
AtGen_B-3_1-3-1_REP_1_ATH11441leaves AtGen_B-3_1-3-1_REP1_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST, harvested 1 hour later
AtGen_B-40_3-5-4_REP3_ATH11478leaves AtGen_B-40_3-5-4_REP3_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST-NPP1, harvested 4 hours later.
AtGen_B-41_3-6-4_REP3_ATH11479leaves AtGen_B-41_3-6-4_REP3_ATH1.CEL
Treatment: leaves infiltrated with 1 µM Flg-22, harvested 4 hours later.
AtGen_B-42_3-7-4_REP3_ATH11480leaves AtGen_B-42_3-7-4_REP3_ATH1.CEL
Treatment: leaves infiltrated with 100µg/ml LPS from P.syringae pv.tomato DC3000, harvested 4 hours later
AtGen_B-4_1-4-1_REP_1_ATH11442leaves AtGen_B-4_1-4-1_REP1_ATH1.CEL
Treatment: leaves infiltrated with 10 µM HrpZ, harvested 1 hour later
AtGen_B-5_1-5-1_REP_1_ATH11443leaves AtGen_B-5_1-5-1_REP1_ATH1.CEL
Treatment: leaves infiltrated with 1 µM GST-NPP1, harvested 1 hour later
AtGen_B-6_1-6-1_REP_1_ATH11444leaves AtGen_B-6_1-6-1_REP1_ATH1.CEL
Treatment: leaves infiltrated with 1 µM Flg-22, harvested 1 hour later
AtGen_B-7_1-7-1_REP_1_ATH11445leaves AtGen_B-7_1-7-1_REP1_ATH1.CEL
Treatment: leaves infiltrated with 100µg/ml LPS from P.syringae pv.tomato DC3000, harvested 1 hour later.
AtGen_B-8_1-1-4_REP_1_ATH11446leaves AtGen_B-8_1-1-4_REP1_ATH1.CEL
Treatment: leaves infiltrated with water, harvested 4 hours later.
AtGen_B-9_1-2-4_REP_1_ATH11447leaves AtGen_B-9_1-2-4_REP1_ATH1.CEL
Treatment: leaves infiltrated with 1 mM CaCl2 + 2.5 mM MgCl2, harvested 4 hours later.