NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Experiment:120
Title:AtGenExpress:Response to virulent, avirulent, typeIII-secretion system deficient and nonhost bacter
Date:2004-07-09
Description:AtGenExpress: A multinational coordinated effort to uncover the transcriptome of the multicellular model organism Arabidopsis thaliana (Hybridisations done at NASC)The activity of genes and their encoded products can be regulated in several ways, but transcription is the primary level, since all other modes of regulation (RNA splicing, RNA and protein stability, etc.) are dependent on a gene being transcribed in the first place. The importance of transcriptional regulation has been underscored by the recent flood of global expression analyses, which have confirmed that transcriptional co-regulation of genes that act together is the norm, not the exception. Moreover, many studies suggest that evolutionary change is driven in large part by modifications of transcriptional programs. An essential first step toward deciphering the transcriptional code is to determine the expression pattern of all genes. With this goal in mind, an international effort to develop a gene expression atlas of Arabidopsis has been underway since fall 2003. This project, dubbed AtGenExpress, is funded by the DFG, and will provide the Arabidopsis community with access to a large set of Affymetrix microarray data. As part of this collaboration, we have generated expression data from 80 biologicaly different samples in triplicate. Series 2: Base line experiment for pathogen infection. Growth and infection conditions: Seeds will be stratified for 3 days at 4°C and sown on soil. Plants are grown at 22°C under a 8/16 hour light/dark regime in Percival growth chambers. All pathogen treatments will be performed on leaves of 5-weeks old plants. Bacterial infiltrations will be performed with 10-8 cfu/ml in 10 mM MgCl2. 5x105 spores of Phytophthora infestans in water will be applied to leaf surfaces. LPS (100 µg/ml), HrpZ (1 µM), NPP1 (2 µM) or flagellin (1 µM flg22) will be applied in water. Experiments should be performed in triplicate at the time points indicated below.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
AtGen_A-10_22-2_REP2_ATH11398leaves AtGen_A-10_22-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-11_22-3_REP3_ATH11399leaves AtGen_A-11_22-3_REP3_ATH1.CEL
Treatment: "infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-13_23-1_REP1_ATH11400leaves AtGen_A-13_23-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-14_23-2_REP2_ATH11401leaves AtGen_A-14_23-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-16_23-4_REP3_ATH11402leaves AtGen_A-16_23-4_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-17_24-1_REP1_ATH11403leaves AtGen_A-17_24-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-18_24-2_REP2_ATH11404leaves AtGen_A-18_24-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-19_24-3_REP3_ATH11405leaves AtGen_A-19_24-3_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-1_17-1_REP1_ATH11391leaves AtGen_A-1_17-1_REP1_ATH1.CEL
Treatment: untreated, harvested at 0h
AtGen_A-21_25-1_REP1_ATH11406leaves AtGen_A-21_25-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-23_25-3_REP2_ATH11407leaves AtGen_A-23_25-3_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-24_25-4_REP3_ATH11408leaves AtGen_A-24_25-4_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-25_26-1_REP1_ATH11409leaves AtGen_A-25_26-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-26_26-2_REP2_ATH11410leaves AtGen_A-26_26-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-28_26-4_REP3_ATH11411leaves AtGen_A-28_26-4_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato avrRpm1, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-29_27-1_REP1_ATH11412leaves AtGen_A-29_27-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-2_17-2_REP2_ATH11392leaves AtGen_A-2_17-2_REP2_ATH1.CEL
Treatment: untreated, harvested at 0h
AtGen_A-30_27-2_REP2_ATH11413leaves AtGen_A-30_27-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-31-27-3_REP3_ATH11414leaves AtGen_A-31_27-3_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-33_28-1_REP1_ATH11415leaves AtGen_A-33_28-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested fter 6h
AtGen_A-34_28-2_REP2_ATH11416leaves AtGen_A-34_28-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested fter 6h
AtGen_A-35_28-3_REP3_ATH11417leaves AtGen_A-35_28-3_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested fter 6h
AtGen_A-37_29-1_REP1_ATH11418leaves AtGen_A-37_29-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-38_29-2_REP2_ATH11419leaves AtGen_A-38_29-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-3_17-3_REP3_ATH11393leaves AtGen_A-3_17-3_REP3_ATH1.CEL
Treatment: untreated, harvested at 0h
AtGen_A-40_29-4_REP3_ATH11420leaves AtGen_A-40_29-4_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000 hrcC-, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-41_30-1_REP1_ATH11421leaves AtGen_A-41_30-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-42_30-2_REP2_ATH11422leaves AtGen_A-42_30-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-43_30-3_REP3_ATH11423leaves AtGen_A-43_30-3_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-45_31-1_REP1_ATH11424leaves AtGen_A-45_31-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-46_31-2_REP2_ATH11425leaves AtGen_A-46_31-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-48_31-4_REP3_ATH11426leaves AtGen_A-48_31-4_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-49_32-1_REP1_ATH11427leaves AtGen_A-49_32-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-50_32-2_REP2_ATH11428leaves AtGen_A-50_32-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-52_32-4_REP3_ATH11429leaves AtGen_A-52_32-4_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv phaseolicola, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-53_33-1_REP1_ATH11430leaves AtGen_A-53_33-1_REP1_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-54_33-2_REP2_ATH11431leaves AtGen_A-54_33-2_REP2_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-55_33-3_REP3_ATH11432leaves AtGen_A-55_33-3_REP3_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-58_34-2_REP1_ATH11433leaves AtGen_A-58_34-2_REP1_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-59_34-3_REP2_ATH11434leaves AtGen_A-59_34-3_REP2_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-5_21-1_REP1_ATH11394leaves AtGen_A-5_21-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-60_34-4_REP3_ATH11435leaves AtGen_A-60_34-4_REP3_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 6h
AtGen_A-61_35-1_REP1_ATH11436leaves AtGen_A-61_35-1_REP1_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-62_35-2_REP2_ATH11437leaves AtGen_A-62_35-2_REP2_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-64_35-4_REP3_ATH11438leaves AtGen_A-64_35-4_REP3_ATH1.CEL
Treatment: infiltrated with 10 mM MgCl2, control, 2 leaves per plant, 8 plants pooled, harvested after 24h
AtGen_A-6_21-2_REP2_ATH11395leaves AtGen_A-6_21-2_REP2_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-8_21-4_REP3_ATH11396leaves AtGen_A-8_21-4_REP3_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 2h
AtGen_A-9_22-1_REP1_ATH11397leaves AtGen_A-9_22-1_REP1_ATH1.CEL
Treatment: infiltrated with 1x108 cfu/ml Pseudomonas syringae pv tomato DC3000, 2 leaves per plant, 8 plants pooled, harvested after 6h