NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Molecular bases of zinc tolerance and accumulation by Arabidopsis halleri
Description:This application is from a NERC-funded consortium (Mark MacNair, Nick Smirnoff, Exeter) and (Brian Ford-Lloyd, John Newbury, Birmingham). Metal tolerance is one of the classic examples of micro-evolution. Despite extensive research the physiological bases of the adaptation in plants are largely unknown. Arabidopsis halleri is a zinc tolerant, zinc accumulating species whereas Arabidopsis petraea is non-accumulating and non-tolerant. The objective of our programme is to identify a) those key genes that act to determine Zn tolerance and accumulation in Arabidopsis (and which account for the difference in performance of A. halleri and A. petraea grown in the presence of elevated Zn) and b) those _downstream_ genes that are expressed as part of the tolerance or accumulation response.Phase 1: Total of 24 chips: Material ready by May 2003 The results will a) tell us how effectively material derived from other Arabidopsis species hybridises to the chips, and b) identify genes that are differentially expressed in the two species in the presence and absence of Zn stress (thus providing initial lists of genes that may be responsible for Zn tolerance or accumulation- but see phase 2)· A. halleri exposed to low and high Zn; root and leaf mRNAs extracted: 3 replicates of each: = 12 slides· A. petraea exposed to low and high Zn; root and leaf mRNAs extracted: 3 replicates of each: = 12 slidesPhase 2: Total of 48 chips: Material ready by September 2003The results will tell us which genes, identified as having appropriate expression patterns, co-segregate with the Zn tolerance or accumulation phenotype and will provide firmer candidate genes for intensive study. Bulks will be produced from F3 progeny (from the halleri x petraea cross) following phentoypic analyses for Zn tolerance and accumulation.· A bulk of F3 progeny all exhibiting high Zn tolerance: exposed to low and high Zn; leaf and root mRNAs: 3 replicates: = 12 slides· A bulk of F3 progeny all exhibiting low Zn tolerance: exposed to low and high Zn; leaf and root mRNAs: 3 replicates: = 12 slides· A bulk of F3 progeny all exhibiting high Zn accumulation: exposed to low and high Zn; leaf and root mRNAs: 3 replicates: = 12 slides· A bulk of F3 progeny all exhibiting low Zn accumulation: exposed to low and high Zn; leaf and root mRNAs: 3 replicates: = 12 slides
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Newbury_2-10_non-acc-bulk-highZn-leaf(NLH)_Rep1_ATH11081Rosette leaves Newbury_2-10_non-acc-bulk-highZn-leaf(NLH)_Rep1_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-11_non-acc-bulk-highZn-leaf(NLH)_Rep2_ATH11082Rosette leaves Newbury_2-11_non-acc-bulk-highZn-leaf(NLH)_Rep2_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-12_non-acc-bulk-highZn-leaf(NLH)_Rep3_ATH11083Rosette leaves Newbury_2-12_non-acc-bulk-highZn-leaf(NLH)_Rep3_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-13_acc-bulk-control-root(ARO)_Rep1_ATH11084whole root system Newbury_2-13_acc-bulk-control-root(ARO)_Rep1_ATH1.CEL
Newbury_2-14_acc-bulk-control-root(ARO)_Rep2_ATH11085whole root system Newbury_2-14_acc-bulk-control-root(ARO)_Rep2_ATH1.CEL
Newbury_2-15_acc-bulk-control-root(ARO)_Rep3_ATH11086whole root system Newbury_2-15_acc-bulk-control-root(ARO)_Rep3_ATH1.CEL
Newbury_2-16_acc-bulk-highZn-root(ARH)_Rep1_ATH11087whole root system Newbury_2-16_acc-bulk-highZn-root(ARH)_Rep1_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-17_acc-bulk-highZn-root(ARH)_Rep2_ATH11088whole root system Newbury_2-17_acc-bulk-highZn-root(ARH)_Rep2_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-18_acc-bulk-highZn-root(ARH)_Rep3_ATH11089whole root system Newbury_2-18_acc-bulk-highZn-root(ARH)_Rep3_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-19_acc-bulk-control-leaf(ALO)_Rep1_ATH11090Rosette leaves Newbury_2-19_acc-bulk-control-leaf(ALO)_Rep1_ATH1.CEL
Newbury_2-1_non-acc-bulk-control-root(NRO)_Rep1_ATH11072whole root system Newbury_2-1_non-acc-bulk-control-root(NRO)_Rep1_ATH1.CEL
Newbury_2-20_acc-bulk-control-leaf(ALO)_Rep2_ATH11091Rosette leaves Newbury_2-20_acc-bulk-control-leaf(ALO)_Rep2_ATH1.CEL
Newbury_2-21_acc-bulk-control-leaf(ALO)_Rep3_ATH11092Rosette leaves Newbury_2-21_acc-bulk-control-leaf(ALO)_Rep3_ATH1.CEL
Newbury_2-22_acc-bulk-highZn-leaf(ALH)_Rep1_ATH11093Rosette leaves Newbury_2-22_acc-bulk-highZn-leaf(ALH)_Rep1_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-23_acc-bulk-highZn-leaf(ALH)_Rep2_ATH11094Rosette leaves Newbury_2-23_acc-bulk-highZn-leaf(ALH)_Rep2_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-24_acc-bulk-highZn-leaf(ALH)_Rep3_ATH11095Rosette leaves Newbury_2-24_acc-bulk-highZn-leaf(ALH)_Rep3_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-2_non-acc-bulk-control-root(NRO)_Rep2_ATH11073whole root system Newbury_2-2_non-acc-bulk-control-root(NRO)_Rep2_ATH1.CEL
Newbury_2-3_non-acc-bulk-control-root(NRO)_Rep3_ATH11074whole root system Newbury_2-3_non-acc-bulk-control-root(NRO)_Rep3_ATH1.CEL
Newbury_2-4_non-acc-bulk-highZn-root(NRH)_Rep1_ATH11075whole root system Newbury_2-4_non-acc-bulk-highZn-root(NRH)_Rep1_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-5_non-acc-bulk-highZn-root(NRH)_Rep2_ATH11076whole root system Newbury_2-5_non-acc-bulk-highZn-root(NRH)_Rep2_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-6_non-acc-bulk-highZn-root(NRH)_Rep3_ATH11077whole root system Newbury_2-6_non-acc-bulk-highZn-root(NRH)_Rep3_ATH1.CEL
Treatment: 25um Zinc
Newbury_2-7_non-acc-bulk-control-leaf(NLO)_Rep1_ATH11078Rosette leaves Newbury_2-7_non-acc-bulk-control-leaf(NLO)_Rep1_ATH1.CEL
Newbury_2-8_non-acc-bulk-control-leaf(NLO)_Rep2_ATH11079Rosette leaves Newbury_2-8_non-acc-bulk-control-leaf(NLO)_Rep2_ATH1.CEL
Newbury_2-9_non-acc-bulk-control-leaf(NLO)_Rep3_ATH11080Rosette leaves Newbury_2-9_non-acc-bulk-control-leaf(NLO)_Rep3_ATH1.CEL