NASCArrays Information at The BAR
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the
experiment number. To download the CEL files, please click on the ftp link.
| Experiment: | 108 |
| Title: | Circadian expression of genes:modelling the Arabidopsis circadian clock |
| Date: | 2004-12-01 |
| Description: | Our aim is to study the circadian expression of genes to aid in our attempt of modelling the Arabidopsis circadian clock. Circadian microarray data have previously been published for plants after white light (WL)-dark cycles, using the 8k chip (Harmer et al. 2000). We intend to repeat this experiment using the 26k chips and are coordinating with Dr. Harmer, who is pursuing complementary experiments in UC Davis. Plants will be transferred to continuous WL after entrainment to 12h:12h light dark cycles. RNAs will be harvested every 4 hours over two days, with the same accession and sampling intervals used previously by Harmer et al. The two days of sampling provide internal replication. Our experience shows that this is the most economical design: it is easier to identify rhythms over a two-day timecourse than in two replicates of a single day. Hence: 13 RNA samples on 13 chips in total.METHOD:Seed was sown on MS agar plates with 3% sucrose, imbibed at 4 C for 96 hours. Seed was then entrained for 7 days at 22C, in cycles of 12 hours white light, 12 hours darkness. After 7 days they were transferred to constant white light at 22 C: this is time 0h. Tissue harvested at the time points shown after time 0. |
| ftp Link: | ftp Link |
| Slide ID | Slide Name | Genetic Background | Tissue | Stock Code | Cel File |
|---|---|---|---|---|---|
| Edwards_1-10_62hr_Rep1_ATH1 | 1068 | whole seedlings | N1093 | KE001_ATH1_A10-edwards-62.CEL | |
| Edwards_1-11_66hr_Rep1_ATH1 | 1069 | whole seedlings | N1093 | KE001_ATH1_A11-edwards-66.CEL | |
| Edwards_1-12_70hr_Rep1_ATH1 | 1070 | whole seedlings | N1093 | KE001_ATH1_A12-edwards-70.CEL | |
| Edwards_1-13_74hr_Rep1_ATH1 | 1071 | whole seedlings | N1093 | KE001_ATH1_A13-edwards-74.CEL | |
| Edwards_1-1_26hr_Rep1_ATH1 | 1059 | whole seedlings | N1093 | KE001_ATH1_A1-edwards-26.CEL | |
| Edwards_1-2_30hr_Rep1_ATH1 | 1060 | whole seedlings | N1093 | KE001_ATH1_A2-edwards-30.CEL | |
| Edwards_1-3_34hr_Rep1_ATH1 | 1061 | whole seedlings | N1093 | KE001_ATH1_A3-edwards-34.CEL | |
| Edwards_1-4_38hr_Rep1_ATH1 | 1062 | whole seedlings | N1093 | KE001_ATH1_A4-edwards-38.CEL | |
| Edwards_1-5_42hr_Rep1_ATH1 | 1063 | whole seedlings | N1093 | KE001_ATH1_A5-edwards-42.CEL | |
| Edwards_1-6_46hr_Rep1_ATH1 | 1064 | whole seedlings | N1093 | KE001_ATH1_A6-edwards-46.CEL | |
| Edwards_1-7_50hr_Rep1_ATH1 | 1065 | whole seedlings | N1093 | KE001_ATH1_A7-edwards-50.CEL | |
| Edwards_1-8_54hr_Rep1_ATH1 | 1066 | whole seedlings | N1093 | KE001_ATH1_A8-edwards-54.CEL | |
| Edwards_1-9_58hr_Rep1_ATH1 | 1067 | whole seedlings | N1093 | KE001_ATH1_A9-edwards-58.CEL |