NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:Differential gene expression patterns in the phosphate deficient mutant, pho 1
Description:Background: The UK horticultural and agricultural industries routinely apply large amounts of inorganic fertiliser to maintain crop yield and quality, since chemical assays of soil nutrients are unreliable. Excessive fertiliser applications are costly and can lead to unnecessary pollution. A possible solution is to use sensor (GM or non-GM) technologies that exploit the changes in plant gene expression under incipient nutrient deficiency.Aim: The aim of this project is to use mutants with reduced leaf phosphate contents to identify genes upregulated in response to phosphate stress. Preliminary gene expression analysis has identified several phosphate responsive genes to be upregulated in the pho1 mutant. However, further replicates of the experiment are required to confirm these changes.Methods: Arabidopsis mutant pho1 (N8507) and its parent ecotype Columbia 2 (N907) will be grown on MS agar under identical conditions. RNA will be extracted from the rosette leaves of both parent and mutant and the same growth stage. By comparing the expression profiles, we will be able to differentiate between genes that are upregulated in leaves experiencing phosphate stress. Previously, two GeneChips have been used (mutant and parent) to provide preliminary data. A further two biological replicates are now required to confirm these results. Promoters and transcripts of these genes will underpin the development of novel sensor technologies, and knowledge of the gene expression profiles will improve our understanding of the physiology of plant mineral nutrition.
ftp Link:ftp Link

Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
Hammond_2-1_Col2wildtype_Rep1_ATH1759Rosette leaves N907Hammond_2-1_Col2wildtype_Rep1_ATH1.cel
Hammond_2-2_pho1mutant_Rep1_ATH1760Col-2Rosette leaves N8507Hammond_2-2_pho1mutant_Rep1_ATH1.cel
Hammond_2-3_Col2wildtype_Rep2_ATH11005Rosette leaves N907Hammond_2-3_Col2wildtype_Rep2_ATH1.cel
Hammond_2-4_pho1mutant_Rep2_ATH11006Col-2Rosette leaves N8507Hammond_2-4_pho1mutant_Rep2_ATH1.cel
Hammond_2-5_Col2wildtype_Rep3_ATH11007Rosette leaves N907Hammond_2-5_Col2wildtype_Rep3_ATH1.cel
Hammond_2-6_pho1mutant_Rep3_ATH11008Col-2Rosette leaves N8507Hammond_2-6_pho1mutant_Rep3_ATH1.cel