NASCArrays Information at The BAR

Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed from text files available on the NASCArrays site. NASCArrays data is on iPlant server. To download experiment data from iPlant, please click on the experiment number. To download the CEL files, please click on the ftp link.

Title:The molecular basis of plant insect interactions
Description:The aim of this study is to identify Arabidopsis genes whose expression is altered by aphid feeding. An understanding of the plant aphid interaction at the level of the plant transcriptome will 1) consolidate current areas of investigation focused on the phloem composition (the aphid diet), 2) open up areas of plant aphid interactions for ourselves and other workers, 3) Contribute to understanding the use of new molecular technologies in an environmental context and 4) contribute to existing and development of novel control strategies.Our Arabidopsis/Myzus persicae system provides a valuable model for the study because of: a) the advantages of using Arabidopsis, b) The ability to use clonal insects, c) phloem feeding aphids facilitate focus on a specific cell type, d) aphid stylectomy allows collection of pure phloem sap to monitor phloem phenotype of the plant and the insect diet, e) we have techniques to monitor the reproductive performance and feeding behaviour aphids.Our strategy has been to test the function of selected genes, particularly those regulating phloem composition (the feeding site of the aphid) based on current phloem models of phloem function. Gene choice is limited the simplicity of current models of phloem aphid interaction.We propose a simple two treatment (aphid infested vs control plants) experiment that will identify novel target genes for future analysis. Arabidopsis plants (variety Columbia) will be grown in 16/8 light/dark in temperature controlled growth rooms. At growth stage 3.90, when rosette growth is complete, 10 clonal adult Myzus persicae will be caged in clip cages on the two largest leaves on each plant. Control plants will be treated identically except that the cages will be empty. Leaves will be harvested 8 h after infestation. This time point is selected as we know that 90% of aphids are plugged into the sieve element within 2h and that a 6h lag phase has period has previously been used when examining gene expression affected by wounding. In subsequent experiments we will examine time courses of expression of relevant genes using other approaches. Pooling two leaves from each of ten plants will generate the RNA sample, ensuring that expression signals are representative of the population of plants.
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Slide Information:
Slide IDSlide NameGenetic BackgroundTissueStock CodeCel File
JPritchard_A-1_CTR_Rep1_ATH19992 mature rosette leaves N3176JPritchard_A-1_CTR_Rep1_ATH1.cel
JPritchard_A-2_CTR_Rep2_ATH110002 mature rosette leaves N3176JPritchard_A-2_CTR_Rep2_ATH1.cel
JPritchard_A-3_CTR_Rep3_ATH110012 mature rosette leaves N3176JPritchard_A-3_CTR_Rep3_ATH1.cel
JPritchard_A-4_API_Rep1_ATH110022 mature rosette leaves N3176JPritchard_A-4_API_Rep1_ATH1.cel
JPritchard_A-5_API_Rep2_ATH110032 mature rosette leaves N3176JPritchard_A-5_API_Rep2_ATH1.cel
JPritchard_A-6_API_Rep3_ATH110042 mature rosette leaves N3176JPritchard_A-6_API_Rep3_ATH1.cel