| Description: | The C-line contains additional copies of chalcone synthase (CHS) and these result in silencing of the endogenous copy at tt4. The clv1-2 mutation was introduced into this line as a second marker. The line was mutagenised and several revertants obtained which show reduced silencing. The best characterised of these is hog1. The hog1 mutant shows reduced DNA methylation and more intact CHS transcript in Northern blots. I have been funded by the BBSRC gene flow initiative to look for downstream targets of DNA methylation by looking for genes which are up or down regulated by hog1. Three week old plants of the genotype C/C clv1-2 with or without the hog1 mutation will be used to prepare RNA for the study. I will take Sean's advice of the best isolation method to use. I have set the sample size at two slides for each sample. I am not sure if this will be enough and again I would be grateful for advice once Sean has the pilot work done. My lab and culture room refurbishment is nearly complete and I should shortly be in a position to grow the plants and make the RNA. |
NASCArrays Information at The BAR
Welcome to NASCArrays information at the BAR. This page hosts meta-information from the NASCArrays service (2002-2013). This information was parsed
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experiment number. To download the CEL files, please click on the ftp link.